From May 13 to 14, the "CryoET Workshop: Tissue Sample Extraction and Lamella Preparation" was held at the Multimodal Cross-Scale Biomedical Imaging Facility in Beijing's Huairou Science City. Organized by the Cryo-Electron Microscopy Subcommittee of the Biophysical Society of China and hosted by the Institute of Biophysics, Chinese Academy of Sciences, the event gathered 19 participants from 15 universities and research institutions.

The workshop opened with an introduction by Li Shuoguo, a senior engineer at the Bioimaging Center, who detailed the facility's capabilities and technical support. This was followed by an overview of Thermo Fisher's cryo-electron microscopy solutions by engineer Zhang Shiying. Participants toured the facility, gaining insights into its advanced hardware and potential for expanding CryoET applications.

Practical sessions began with a training on the Helios 5 Hydra CX plasma-focused ion beam scanning electron microscope by Thermo Fisher engineer Zhen Wenxuan. The training covered sample extraction protocols and optimization strategies, sparking lively discussions.

On May 14, the hands-on phase continued with guidance from engineers Zeng Yan, Li Zhongwen, and Wang Tianjiao, along with Thermo Fisher's Zhen Wenxuan and Ma Rui. Participants practiced the complete CryoET workflow, addressing challenges like cryogenic contamination control and data collection efficiency.

The workshop offered practical solutions for tissue sample thinning and facilitated collaboration among CryoET professionals. Participants left with actionable insights, having resolved technical issues that previously hindered their research.

Concluding with applause, the event marked a successful exchange of knowledge. The Cryo-Electron Microscopy Subcommittee plans to continue offering high-quality training and workshops, fostering talent development and scientific breakthroughs in China's cryo-electron microscopy field.